Electrophoretic mobility shift as a molecular beacon-based readout for miRNA detection.

ABSTRACT: MicroRNAs are short, non-coding RNA sequences involved in gene expression regulation. Quantification of miRNAs in biological fluids involves time consuming and laborious methods such as Northern blotting or PCR-based techniques. Molecular beacons (MB) are an attractive means for rapid detection of miRNAs, although the need for sophisticated readout methods limits their use in research and clinical settings. Here, we introduce a novel method based on delayed electrophoretic mobility, as a quantitative means for detection of miRNAs-MB hybridization. Upon hybridization with the target miRNAs, MB form a fluorescent duplex with reduced electrophoretic mobility, thus bypassing the need for additional staining. In addition to emission of light, the location of the fluorescent band on the gel acts

SUBMITTER: Oliveira-Jr GP 

PROVIDER: S-EPMC8461749 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature