Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis.

ABSTRACT: Ligand-dependent biosensors are valuable tools for coupling the intracellular concentrations of small molecules to easily detectable readouts such as absorbance, fluorescence, or cell growth. While ligand-dependent biosensors are widely used for monitoring the production of small molecules in engineered cells and for controlling or optimizing biosynthetic pathways, their application to directed evolution for biocatalysts remains underexplored. As a consequence, emerging continuous evolution technologies are rarely applied to biocatalyst evolution. Here, we develop a panel of ligand-dependent biosensors that can detect a range of small molecules. We demonstrate that these biosensors can link enzymatic activity to the production of an essential phage protein to enable biocatalyst-dependent p

SUBMITTER: Jones KA 

PROVIDER: S-EPMC8461764 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature