Large dependency of intracellular NAD and CoA pools on cultivation conditions in Saccharomyces cerevisiae.

ABSTRACT:

Objective

The objective of this study was to investigate the variation of NAD and CoA metabolite pools in Saccharomyces cerevisiae cultivated under various cultivation conditions. This study complements a previous report on glycolytic, pentose phosphate pathway, tricarboxylic acid cycle, amino acids, and deoxy-/nucleoside phosphate pools determined under the same cultivation conditions.

Results

S. cerevisiae pellets from batch (four carbohydrate sources) and chemostat (carbon-, nitrogen-, phosphate-limited and a range of dilution rates) bioreactor cultivations were extracted and analyzed with two recently established absolute quantitative liquid chromatography mass spectrometry (LC-MS/MS) methods for NAD and CoA metabolites. Both methods apply ¹³C internal standar

SUBMITTER: Kumar K 

PROVIDER: S-EPMC8461857 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature