Intracolic ultrasound molecular imaging: a novel method for assessing colonic tumor necrosis factor-α expression in inflammatory bowel disease.

ABSTRACT:

Background

While anti-tumor necrosis factor alpha (TNF-α) therapy has been proven effective in inflammatory bowel disease (IBD), approximately 40% of patients lose the response. Transmembrane TNF-α (mTNF-α) expression in the intestinal mucosa is correlated with therapeutic efficacy, and quantification of mTNF-α expression is significant for predicting response. However, conventional intravenous application of microbubbles is unable to assess mTNF-α expression in intestinal mucosa. Herein, we proposed intracolic ultrasound molecular imaging with TNF-α-targeted microbubbles (MB_TNF-α) to quantitatively detect mTNF-α expression in the intestinal mucosa.

Methods

MB_TNF-α was synthesized via a biotin-streptavidin bridging method. TNF-α-targeted ultrasound imag

SUBMITTER: Miao X 

PROVIDER: S-EPMC8461918 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature