Self-organising human gonads generated by a Matrigel-based gradient system.

ABSTRACT:

Background

Advances in three-dimensional culture technologies have led to progression in systems used to model the gonadal microenvironment in vitro. Despite demonstrating basic functionality, tissue organisation is often limited. We have previously detailed a three-dimensional culture model termed the three-layer gradient system to generate rat testicular organoids in vitro. Here we extend the model to human first-trimester embryonic gonadal tissue.

Results

Testicular cell suspensions reorganised into testis-like organoids with distinct seminiferous-like cords situated within an interstitial environment after 7 days. In contrast, tissue reorganisation failed to occur when mesonephros, which promotes testicular development in vivo, was included in the tissue digest. Organoid

SUBMITTER: Oliver E 

PROVIDER: S-EPMC8461962 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature