Large-scale data analysis for robotic yeast one-hybrid platforms and multi-disciplinary studies using GateMultiplex.

ABSTRACT:

Background

Yeast one-hybrid (Y1H) is a common technique for identifying DNA-protein interactions, and robotic platforms have been developed for high-throughput analyses to unravel the gene regulatory networks in many organisms. Use of these high-throughput techniques has led to the generation of increasingly large datasets, and several software packages have been developed to analyze such data. We previously established the currently most efficient Y1H system, meiosis-directed Y1H; however, the available software tools were not designed for processing the additional parameters suggested by meiosis-directed Y1H to avoid false positives and required programming skills for operation.

Results

We developed a new tool named GateMultiplex with high computing performance using C++.

SUBMITTER: Tsai NC 

PROVIDER: S-EPMC8461970 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature