Identification and Functional Characterization of a Novel Androgen Receptor Coregulator, EAP1.

ABSTRACT: The androgen receptor (AR) plays an essential role in the development of prostate cancer, and androgen-deprivation therapy is used as a first-line treatment for prostate cancer. However, under androgen-deprivation therapy, castration-resistant prostate cancer inevitably arises, suggesting that the interacting transcriptional coregulators of AR are promising targets for developing novel therapeutics. In this study, we used novel proteomic techniques to evaluate the AR interactome, including biochemically labile binding proteins, which might go undetected by conventional purification methods. Using rapid immunoprecipitation mass spectrometry of endogenous proteins, we identified enhanced at puberty 1 (EAP1) as a novel AR coregulator, whereas its interaction with AR could not be detected unde

SUBMITTER: Yokoyama A 

PROVIDER: S-EPMC8462380 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature