The cohesin stabilizer Sororin drives G₂-M transition and spindle assembly in mammalian oocytes.

ABSTRACT: During the S phase of mitosis, Sororin is recruited by acetylated Smc3 and stabilizes sister chromatid cohesion by counteracting the Wapl-Pds5 interaction. Thereafter, Sororin is phosphorylated during prophase and translocated to the cytoplasm, where its function remains poorly understood. Here, we report that Sororin acts as a regulator of meiotic G₂-M transition and spindle assembly in mammalian oocytes. Sororin is present in the nucleus of GV oocytes and becomes associated with the spindle apparatus during meiosis I in mice. Depletion of Sororin causes failure of GVBD due to inactivation of Cdk1 and defective spindle assembly because of reduced levels of Cyclin B2. We validate Sororin interactions with Cyclin B2 that protects it from destruction by APC^Cdh1, which d

SUBMITTER: Zhou C 

PROVIDER: S-EPMC8462903 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature