UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection.
Ontology highlight
ABSTRACT:
SUBMITTER: Alcantara R
PROVIDER: S-EPMC8463329 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection.
Alcántara Roberto R Peñaranda Katherin K Mendoza-Rojas Gabriel G Nakamoto Jose A JA Dueñas Eva E Alvarez Daniela D Adaui Vanessa V Milón Pohl P
STAR protocols 20210925 4
Here, we describe a detailed step-by-step protocol to detect SARS-CoV-2 RNA using RT-PCR-mediated amplification and CRISPR/Cas-based visualization. The optimized assay uses basic molecular biology equipment such as conventional thermocyclers and transilluminators for qualitative detection. Alternatively, a fluorescence plate reader can be used for quantitative measurements. The protocol detects two regions of the SARS-CoV-2 genome in addition to the human RNaseP sample control. Aiming to reach r ...[more]
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