Multi-Omics Analyses Show Disease, Diet, and Transcriptome Interactions With the Virome.

ABSTRACT:

Background & aims

The gut virome includes eukaryotic viruses and bacteriophages that can shape the gut bacterial community and elicit host responses. The virome can be implicated in diseases, such as irritable bowel syndrome (IBS), where gut bacteria play an important role in pathogenesis. We provide a comprehensive and longitudinal characterization of the virome, including DNA and RNA viruses and paired multi-omics data in a cohort of healthy subjects and patients with IBS.

Methods

We selected 2 consecutive stool samples per subject from a longitudinal study cohort and performed metagenomic sequencing on DNA and RNA viruses after enriching for viral-like particles. Viral sequence abundance was evaluated over time, as well as in the context of diet, bacterial composition and

SUBMITTER: Mihindukulasuriya KA 

PROVIDER: S-EPMC8463486 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature