Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections.

ABSTRACT: Teleost fish skin serves as the first line of defense against pathogens. The interaction between pathogen and host skin determines the infection outcome. However, the mechanism(s) that modulate infection remain largely unknown. A proper tissue culture model that is easier to handle but can quantitatively and qualitatively monitor infection progress may shed some lights. Here, we use striped catfish (Pangasius hypophthalmus) to establish an ex vivo skin explant tissue culture model to explore host pathogen interactions. The skin explant model resembles in vivo skin in tissue morphology, integrity, and immune functionality. Inoculation of aquatic pathogen Aeromonas hydrophila in this model induces epidermal exfoliation along with epithelial cell dissociation and inflammation. We conclude tha

SUBMITTER: Siao RF 

PROVIDER: S-EPMC8463585 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature