Micromotor-based localized electroporation and gene transfection of mammalian cells.

ABSTRACT: Herein, we studied localized electroporation and gene transfection of mammalian cells using a metallodielectric hybrid micromotor that is magnetically and electrically powered. Much like nanochannel-based, local electroporation of single cells, the presented micromotor was expected to increase reversible electroporation yield, relative to standard electroporation, as only a small portion of the cell's membrane (in contact with the micromotor) is affected. In contrast to methods in which the entire membrane of all cells within the sample are electroporated, the presented micromotor can perform, via magnetic steering, localized, spatially precise electroporation of the target cells that it traps and transports. In order to minimize nonselective electrical lysis of all cells within the chambe

SUBMITTER: Wu Y 

PROVIDER: S-EPMC8463876 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature