Activation of TMEM16A Ca²⁺-activated Cl^- channels by ROCK1/moesin promotes breast cancer metastasis.

ABSTRACT:

Introduction

Transmembrane protein 16A (TMEM16A) is a Ca²⁺-activated chloride channel that plays a role in cancer cell proliferation, migration, invasion, and metastasis. However, whether TMEM16A contributes to breast cancer metastasis remains unknown.

Objective

In this study, we investigated whether TMEM16A channel activation by ROCK1/moesin promotes breast cancer metastasis.

Methods

Wound healing assays and transwell migration and invasion assays were performed to study the migration and invasion of MCF-7 and T47D breast cancer cells. Western blotting was performed to evaluate the protein expression, and whole-cell patch clamp recordings were used to record TMEM16A Cl^- currents. A mouse model of breast cancer lung metastasis was generated by in

SUBMITTER: Luo S 

PROVIDER: S-EPMC8463928 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature