Offspring production of ovarian organoids derived from spermatogonial stem cells by defined factors with chromatin reorganization.

ABSTRACT:

Introduction

Fate determination of germline stem cells remains poorly understood at the chromatin structure level.

Objectives

Our research hopes to develop successful offspring production of ovarian organoids derived from spermatogonial stem cells (SSCs) by defined factors.

Methods

The offspring production from oocytes transdifferentiated from mouse SSCs with tracking of transplanted SSCs in vivo, single cell whole exome sequencing, and in 3D cell culture reconstitution of the process of oogenesis derived from SSCs. The defined factors were screened with ovarian organoids. We uncovered extensive chromatin reorganization during SSC conversion into induced germline stem cells (iGSCs) using high throughput chromosome conformation.

Results

We demonstrate successful

SUBMITTER: Luo H 

PROVIDER: S-EPMC8463929 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature