The RNA binding protein Quaking represses splicing of the Fibronectin EDA exon and downregulates the interferon response.

ABSTRACT: Quaking (QKI) controls RNA metabolism in many biological processes including innate immunity, where its roles remain incompletely understood. To illuminate these roles, we performed genome scale transcriptome profiling in QKI knockout cells with or without poly(I:C) transfection, a double-stranded RNA analog that mimics viral infection. Analysis of RNA-sequencing data shows that QKI knockout upregulates genes induced by interferons, suggesting that QKI is an immune suppressor. Furthermore, differential splicing analysis shows that QKI primarily controls cassette exons, and among these events, we noted that QKI silences splicing of the extra domain A (EDA) exon in fibronectin (FN1) transcripts. QKI knockout results in elevated production and secretion of FN1-EDA protein, which is a known ac

SUBMITTER: Liao KC 

PROVIDER: S-EPMC8464043 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature