Ontology highlight
ABSTRACT:
SUBMITTER: Gamper H
PROVIDER: S-EPMC8464047 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Gamper Howard H Mao Yujia Y Masuda Isao I McGuigan Henri H Blaha Gregor G Wang Yuhong Y Xu Shoujun S Hou Ya-Ming YM
Nucleic acids research 20210901 17
Inducing tRNA +1 frameshifting to read a quadruplet codon has the potential to incorporate a non-natural amino acid into the polypeptide chain. While this strategy is being considered for genome expansion in biotechnology and bioengineering endeavors, a major limitation is a lack of understanding of where the shift occurs in an elongation cycle of protein synthesis. Here, we use the high-efficiency +1-frameshifting SufB2 tRNA, containing an extra nucleotide in the anticodon loop, to address this ...[more]