MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling.

ABSTRACT:

Background

Cell invasion is a hallmark of metastatic cancer, leading to unfavorable clinical outcomes. In this study, we established two highly invasive lung cancer cell models (A549-i8 and H1299-i8) and identified mesoderm-specific transcript (MEST) as a novel invasive regulator of lung cancer. We aim to characterize its biological function and clinical significance in lung cancer metastasis.

Methods

Transwell invasion assay was performed to establish high-invasive lung cancer cell model. Immunohistochemistry (IHC) was used to detect MEST expression in tumor tissues. Mass spectrometry and bioinformatic analyses were used to identify MEST-regulated proteins and binding partners. Co-immunoprecipitation assay was performed to detect the interaction of MEST and VCP. The biologi

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8464132 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature