Genotyping of Brucella strains isolated from humans and cattle of different geographical regions of Pakistan using MLVA-15.

ABSTRACT:

Background

Prevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. Humans that work with them were also selected for sampling in this study. Total of 600 samples were taken from cattle and humans and subjected to Rose Bengal plate Test (RBPT) for the initial screening of positive samples. Designed primers of B.abortus for cattle and B.melitensis for humans were utilised to perform PCR. Culturing and isolation was carried to further to perform MLVA genotyping assay through the selection of two panels of primer markers.

Results

RBPT showed more numb

SUBMITTER: Akhtar R 

PROVIDER: S-EPMC8464260 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature