Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage-free systems.

ABSTRACT: Stress factors during poultry production can evoke changes in gene transcription and protein synthesis in the hen oviduct and could affect the internal and external egg quality. Studies of relative gene expression require the identification of the most stable reference genes for the quantitative polymerase chain reaction (qPCR) to investigate the reproductive tissues' response in laying hens kept in different production systems. The objective of this study was to determine the most stable reference genes of the magnum tissues of laying hens housed in two different production systems. Hy-Line Brown hens were reared under the same sanitary conditions until 15 weeks of age. Later on, they were transferred into two different production systems, conventional cage (CC) and cage free (CF), up to

SUBMITTER: Rodriguez-Hernandez R 

PROVIDER: S-EPMC8464264 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature