Stability and Validation of a High-Throughput LC-MS/MS Method for the Quantification of Cefepime, Meropenem, and Piperacillin and Tazobactam in Serum.

ABSTRACT:

Background

The class of antibiotics known as β-lactams are a commonly used due to their effectiveness and safety. Therapeutic drug monitoring has been proposed but requires an accurate assay along with well-characterized preanalytic stability, as β-lactams are known to be relatively unstable.

Methods

A high-throughput LC-MS/MS assay validation and stability study was performed for cefepime, meropenem, and piperacillin and tazobactam in serum. Patient samples, standards, and QCs were crashed with acetonitrile containing internal standard. Following centrifugation, an aliquot of the supernatant was diluted with clinical laboratory reagent water and analyzed by LC-MS/MS.

Results

The assay showed linearity between 0.5 and 60 µg/mL for each analyte. The intra- and interass

SUBMITTER: Bjergum MW 

PROVIDER: S-EPMC8464404 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature