Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation.

ABSTRACT: In recent years, stem cell-derived organoids have become a cell culture standard that is widely used for studying various scientific issues that were previously investigated through animal experiments and using common tumor cell lines. After their initial hype, concerns regarding their standardization have been raised. Here, we aim to provide some insights into our experience in standardizing murine colonic epithelial organoids, which we use as a replacement method for research on inflammatory bowel disease. Considering good scientific practice, we examined various factors that might challenge the design and outcome of experiments using these organoids. First, to analyze the impact of antibiotics/antimycotics, we performed kinetic experiments using ZellShield® and measured the gene express

SUBMITTER: Zur Bruegge TF 

PROVIDER: S-EPMC8464427 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature