Time-dependent changes in placental mRNA expression after delivery due to delayed specimen collection.

ABSTRACT:

Problem

Current scientific guidelines recommend collecting placental specimens within two hours of delivery for gene expression analysis. However, collecting samples in a narrow time window is a challenge in the dynamic and unpredictable clinical setting, so delays in placental specimen collection are possible. The purpose of our analysis was to investigate temporal changes in placental gene expression by longitudinally sampling placentas over a 24 h period.

Method of study

Eight placentas from individuals with uncomplicated, term pregnancies delivered by scheduled cesarean section were collected and sampled following the placental delivery and again at 1, 2, 4, 6, and 24 h post-delivery. At each time point, biopsies of chorionic villous tissue were taken from 3 cotyledons t

SUBMITTER: Freedman AA 

PROVIDER: S-EPMC8464485 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature