Plasma kallikrein predicts primary graft dysfunction after heart transplant.

ABSTRACT:

Background

Primary graft dysfunction (PGD) is the leading cause of early mortality after heart transplant. Pre-transplant predictors of PGD remain elusive and its etiology remains unclear.

Methods

Microvesicles were isolated from 88 pre-transplant serum samples and underwent proteomic evaluation using TMT mass spectrometry. Monte Carlo cross validation (MCCV) was used to predict the occurrence of severe PGD after transplant using recipient pre-transplant clinical characteristics and serum microvesicle proteomic data. Putative biological functions and pathways were assessed using gene set enrichment analysis (GSEA) within the MCCV prediction methodology.

Results

Using our MCCV prediction methodology, decreased levels of plasma kallikrein (KLKB1), a critical regulator o

SUBMITTER: Giangreco NP 

PROVIDER: S-EPMC8464488 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature