Three ParA Dimers Cooperatively Assemble on Type Ia Partition Promoters.

ABSTRACT: Accurate DNA segregation is essential for faithful inheritance of genetic material. In bacteria, this process is mainly ensured by partition systems composed of two proteins, ParA and ParB, and a centromere site. Auto-regulation of Par operon expression is important for efficient partitioning and is primarily mediated by ParA for type Ia plasmid partition systems. For the F-plasmid, four ParA_F monomers were proposed to bind to four repeated sequences in the promoter region. By contrast, using quantitative surface-plasmon-resonance, we showed that three ParA_F dimers bind to this region. We uncovered that one perfect inverted repeat (IR) motif, consisting of two hexamer sequences spaced by 28-bp, constitutes the primary ParA_F DNA binding site. A similar but d

SUBMITTER: Boudsocq F 

PROVIDER: S-EPMC8465637 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature