Molecular Characterization of Laboratory Mutants of Fusarium oxysporum f. sp. niveum Resistant to Prothioconazole, a Demethylation Inhibitor (DMI) Fungicide.

ABSTRACT: Fusarium oxysporum f. sp. niveum (FON) is the causal agent of Fusarium wilt in watermelon, an international growth-limiting pathogen of watermelon cultivation. A single demethylation inhibitor (DMI) fungicide, prothioconazole, is registered to control this pathogen, so the risk of resistance arising in the field is high. To determine and predict the mechanism by which FON could develop resistance to prothioconazole, FON isolates were mutagenized using UV irradiation and subsequent fungicide exposure to create artificially resistant mutants. Isolates were then put into three groups based on the EC₅₀ values: sensitive, intermediately resistant, and highly resistant. The mean EC₅₀ values were 4.98 µg/mL for the sensitive, 31.77 µg/mL for the intermediately

SUBMITTER: Hudson O 

PROVIDER: S-EPMC8466437 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature