Pushing the Boundaries: Forensic DNA Phenotyping Challenged by Single-Cell Sequencing.

ABSTRACT: Single-cell sequencing is a fast developing and very promising field; however, it is not commonly used in forensics. The main motivation behind introducing this technology into forensics is to improve mixture deconvolution, especially when a trace consists of the same cell type. Successful studies demonstrate the ability to analyze a mixture by separating single cells and obtaining CE-based STR profiles. This indicates a potential use of the method in other forensic investigations, like forensic DNA phenotyping, in which using mixed traces is not fully recommended. For this study, we collected single-source autopsy blood from which the white cells were first stained and later separated with the DEPArray™ N×T System. Groups of 20, 10, and 5 cells, as well as 20 single cells, were collected

SUBMITTER: Diepenbroek M 

PROVIDER: S-EPMC8466929 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature