Targeted RNA-Seq Reveals the M. tuberculosis Transcriptome from an In Vivo Infection Model.

ABSTRACT: The study of host-pathogen interactions using in vivo models with intracellular pathogens like Mycobacterium tuberculosis (Mtb) entails technical limitations, such as: (i) Selecting an efficient differential lysis system to enrich the pathogen cells; (ii) obtaining sufficient high-quality RNA; and (iii) achieving an efficient rRNA depletion. Thus, some authors had used flow cytometers to separate infected cells or significantly increase the sequencing depth of host-pathogen RNA libraries to observe the pathogens' gene expression. However, these options carry additional expenses in specialized equipment typically not available for all laboratories. Here, we propose an experimental protocol involving differential cell lysis and a probe-based ribosomal depletion to determine the gene expressi

SUBMITTER: Cornejo-Granados F 

PROVIDER: S-EPMC8467220 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature