Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous Taxa.

ABSTRACT: Culture techniques are vital in both traditional and modern fungal taxonomy. Establishing sexual-asexual links and synanamorphs, extracting DNA and secondary metabolites are mainly based on cultures. However, it is widely accepted that a large number of species are not sporulating in nature while others cannot be cultured. Recent ecological studies based on culture-independent methods revealed these unculturable taxa, i.e., dark taxa. Recent fungal diversity estimation studies suggested that environmental sequencing plays a vital role in discovering missing species. However, Sanger sequencing is still the main approach in determining DNA sequences in culturable species. In this paper, we summarize culture-based and culture-independent methods in the study of ascomycetous taxa. High-through

SUBMITTER: Wijayawardene NN 

PROVIDER: S-EPMC8467358 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature