Dataset Information

Use of PEDOT:PSS/Graphene/Nafion Composite in Biosensors Based on Acetic Acid Bacteria.

ABSTRACT: Immobilization of the biocomponent is one of the most important stages in the development of microbial biosensors. In this study, we examined the electrochemical properties of a novel PEDOT:PSS/graphene/Nafion composite used to immobilize Gluconobacter oxydans bacterial cells on the surface of a graphite screen-printed electrode. Bioelectrode responses to glucose in the presence of a redox mediator 2,6-dichlorophenolindophenol were studied. The presence of graphene in the composite reduced the negative effect of PEDOT:PSS on cells and improved its conductivity. The use of Nafion enabled maintaining the activity of acetic acid bacteria at the original level for 120 days. The sensitivity of the bioelectrode based on G. oxydans/PEDOT:PSS/graphene/Nafion composite was shown to be 22 μA × mM-1 × cm-2 within the linear range of glucose concentrations. The developed composite can be used both in designing bioelectrochemical microbial devices and in biotechnology productions for long-term immobilization of microorganisms.

SUBMITTER: Plekhanova Y

PROVIDER: S-EPMC8467571 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Use of PEDOT:PSS/Graphene/Nafion Composite in Biosensors Based on Acetic Acid Bacteria.

Plekhanova Yulia Y Tarasov Sergei S Reshetilov Anatoly A

Biosensors 20210913 9

Immobilization of the biocomponent is one of the most important stages in the development of microbial biosensors. In this study, we examined the electrochemical properties of a novel PEDOT:PSS/graphene/Nafion composite used to immobilize <i>Gluconobacter oxydans</i> bacterial cells on the surface of a graphite screen-printed electrode. Bioelectrode responses to glucose in the presence of a redox mediator 2,6-dichlorophenolindophenol were studied. The presence of graphene in the composite reduce ...[more]

PMID: 34562922

Similar Datasets

Project description:Wearable mechanical sensors have the potential to transform healthcare by enabling patient monitoring outside of the clinic. A critical challenge in the development of mechanical—e.g., strain—sensors is the combination of sensitivity, dynamic range, and robustness. This work describes a highly sensitive and robust wearable strain sensor composed of three layered materials: graphene, an ultrathin film of palladium, and highly plasticized PEDOT:PSS. The role of the graphene is to provide a conductive, manipulable substrate for the deposition of palladium. When deposited at low nominal thicknesses (~8 nm) palladium forms a rough, granular film which is highly piezoresistive (i.e., the resistance increases with strain with high sensitivity). The dynamic range of these graphene/palladium films, however, is poor, and can only be extended to ~10% before failure. This fragility renders the films incompatible with wearable applications on stretchable substrates. To improve the working range of graphene/palladium strain sensors, a layer of highly plasticized PEDOT:PSS is used as a stretchable conductive binder. That is, the conductive polymer provides an alternative pathway for electrical conduction upon cracking of the palladium film and the graphene. The result was a strain sensor that possessed good sensitivity at low strains (0.001% engineering strain) but with a working range up to 86%. The piezoresistive performance can be optimized in a wearable device by sandwiching the conductive composite between a soft PDMS layer in contact with the skin and a harder layer at the air interface. When attached to the skin of the torso, the patch-like strain sensors were capable of detecting heartbeat (small strain) and respiration (large strain) simultaneously. This demonstration highlights the ability of the sensor to measure low and high strains in a single interpolated signal, which could be useful in monitoring, for example, obstructive sleep apnea with an unobtrusive device. Graphical Abstract

Dataset Information

Use of PEDOT:PSS/Graphene/Nafion Composite in Biosensors Based on Acetic Acid Bacteria.

Publications

Use of PEDOT:PSS/Graphene/Nafion Composite in Biosensors Based on Acetic Acid Bacteria.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets