Cep192, a Novel Missing Link between the Centrosomal Core and Corona in Dictyostelium Amoebae.

ABSTRACT: The Dictyostelium centrosome is a nucleus-associated body with a diameter of approx. 500 nm. It contains no centrioles but consists of a cylindrical layered core structure surrounded by a microtubule-nucleating corona. At the onset of mitosis, the corona disassembles and the core structure duplicates through growth, splitting, and reorganization of the outer core layers. During the last decades our research group has characterized the majority of the 42 known centrosomal proteins. In this work we focus on the conserved, previously uncharacterized Cep192 protein. We use superresolution expansion microscopy (ExM) to show that Cep192 is a component of the outer core layers. Furthermore, ExM with centrosomal marker proteins nicely mirrored all ultrastructurally known centrosomal substructures.

SUBMITTER: Pitzen V 

PROVIDER: S-EPMC8467581 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature