Development of Optimal Digesting Conditions for Microplastic Analysis in Dried Seaweed Gracilaria fisheri.

ABSTRACT: Currently, research on the accumulation of microplastics (MPs) in the marine food web is being highlighted. An accurate and reliable digestion method to extract and isolate MPs from complex food matrices has seldom been validated. This study aimed to compare the efficacy of MP isolation among enzymatic-, oxidative-, and the combination of two digestion methods on red seaweed, Gracilaria fisheri. The dried seaweed sample was digested using three different methods under various conditions using enzymes (cellulase and protease), 30% H2O2, and a combination of enzymes and 30% H2O2. The method possessing the best digestion efficiency and polymer recovery rate of MPs was selected, and its effect on spiked plastic polymer integrity was analyzed by Raman spectroscopy. As a result, the enzymatic me

SUBMITTER: Prihandari R 

PROVIDER: S-EPMC8467988 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature