Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging.

ABSTRACT: Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale. According to the characteristics of STED, a high depletion power is required to obtain a high resolution. However, a high laser power can induce severe phototoxicity and photobleaching, which limits the applications for live cell imaging, especially in two-color STED super-resolution imaging. Therefore, we developed a low-power two-color STED super-resolution microscope with a single supercontinuum white-light laser. Using this system, we achieved low-power two-color super-resolution imaging based on digital enhancement technology. Lateral r

SUBMITTER: Zhang J 

PROVIDER: S-EPMC8468006 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature