A Rapid Method for Performing a Multivariate Optimization of Phage Production Using the RCCD Approach.

ABSTRACT: Bacteriophages can be used in various applications, from the classical approach as substitutes for antibiotics (phage therapy) to new biotechnological uses, i.e., as a protein delivery vehicle, a diagnostic tool for specific strains of bacteria (phage typing), or environmental bioremediation. The demand for bacteriophage production increases daily, and studies that improve these production processes are necessary. This study evaluated the production of a T4-like bacteriophage vB_EcoM-UFV09 (an E. coli-infecting phage with high potential for reducing environmental biofilms) in seven types of culture media (Luria-Bertani broth and the M9 minimal medium with six different carbon sources) employing four cultivation variables (temperature, incubation time, agitation, and multiplicity of

SUBMITTER: Silva J 

PROVIDER: S-EPMC8468216 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature