Modulation of Cell Behavior by 3D Biocompatible Hydrogel Microscaffolds with Precise Configuration.

ABSTRACT: Three-dimensional (3D) micronano structures have attracted much attention in tissue engineering since they can better simulate the microenvironment in vivo. Two-photon polymerization (TPP) technique provides a powerful tool for printing arbitrary 3D structures with high precision. Here, the desired 3D biocompatible hydrogel microscaffolds (3D microscaffold) with structure design referring to fibroblasts L929 have been fabricated by TPP technology, particularly considering the relative size of cell seed (cell suspension), spread cell, strut and strut spacing of scaffold. Modulation of the cell behavior has been studied by adjusting the porosity from 69.7% to 89.3%. The cell culture experiment results reveal that the obvious modulation of F-actin can be achieved by using the 3D microscaffold

SUBMITTER: Zhang WC 

PROVIDER: S-EPMC8469000 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature