LSD1 Facilitates Pro-Inflammatory Polarization of Macrophages by Repressing Catalase.

ABSTRACT: The increased level of hydrogen peroxide accompanies some modes of macrophage specification and is linked to ROS-based antimicrobial activity of these phagocytes. In this study, we show that activation of toll-like receptors with bacterial components such as LPS is accompanied by the decline in transcription of hydrogen peroxide decomposing enzyme-catalase, suppression of which facilitates the polarization of human macrophages towards the pro-inflammatory phenotype. The chromatin remodeling at the CAT promoter involves LSD1 and HDAC1, but activity of the first enzyme defines abundance of the two proteins on chromatin, histone acetylation status and the CAT transcription. LSD1 inhibition prior to macrophage activation with LPS prevents CAT repression by enhancing the LSD1 and interfering wi

SUBMITTER: Sobczak M 

PROVIDER: S-EPMC8469135 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature