Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders.

ABSTRACT: An aptamer is a short sequence of synthetic oligonucleotides which bind to their cognate target, specifically while maintaining similar or higher sensitivity compared to an antibody. The in-vitro selection of an aptamer, applying a conjoining approach of chemistry and molecular biology, is referred as Systematic Evolution of Ligands by Exponential enrichment (SELEX). These initial products of SELEX are further modified chemically in an attempt to make them stable in biofluid, avoiding nuclease digestion and renal clearance. While the modification is incorporated, enough care should be taken to maintain its sensitivity and specificity. These modifications and several improvisations have widened the window frame of aptamer applications that are currently not only restricted to in-vitro syste

SUBMITTER: Basu D 

PROVIDER: S-EPMC8469434 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature