Dataset Information

Antifungal Activity of Extracts, Fractions, and Constituents from Coccoloba cowellii Leaves.

ABSTRACT: Coccoloba cowellii Britton (Polygonaceae, order Caryophyllales) is an endemic and critically endangered plant species that only grows in the municipality of Camagüey, a province of Cuba. A preliminary investigation of its total methanolic extract led to the discovery of promising antifungal activity. In this study, a bioassay-guided fractionation allowed the isolation of quercetin and four methoxyflavonoids: 3-O-methylquercetin, myricetin 3,3',4'-trimethyl ether, 6-methoxymyricetin 3,4'-dimethyl ether, and 6-methoxymyricetin 3,3',4'-trimethyl ether. The leaf extract, fractions, and compounds were tested against various fungi and showed strong in vitro antifungal activity against Cryptococcus neoformans and various Candida spp. with no cytotoxicity (CC50 > 64.0 µg/mL) on MRC-5 SV2 cells, determined by a resazurin assay. A Candida albicans SC5314 antibiofilm assay indicated that the antifungal activity of C. cowellii extracts and constituents is mainly targeted to planktonic cells. The total methanolic extract showed higher and broader activity compared with the fractions and mixture of compounds.

SUBMITTER: Mendez D

PROVIDER: S-EPMC8469486 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Antifungal Activity of Extracts, Fractions, and Constituents from Coccoloba cowellii Leaves.

Méndez Daniel D Escalona-Arranz Julio C JC Pérez Enrique Molina EM Foubert Kenn K Matheeussen An A Tuenter Emmy E Cuypers Ann A Cos Paul P Pieters Luc L

Pharmaceuticals (Basel, Switzerland) 20210910 9

Coccoloba cowellii Britton (Polygonaceae, order Caryophyllales) is an endemic and critically endangered plant species that only grows in the municipality of Camagüey, a province of Cuba. A preliminary investigation of its total methanolic extract led to the discovery of promising antifungal activity. In this study, a bioassay-guided fractionation allowed the isolation of quercetin and four methoxyflavonoids: 3-O-methylquercetin, myricetin 3,3',4'-trimethyl ether, 6-methoxymyricetin ...[more]

PMID: 34577616

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Antifungal Activity of Extracts, Fractions, and Constituents from Coccoloba cowellii Leaves.

Publications

Antifungal Activity of Extracts, Fractions, and Constituents from <i>Coccoloba cowellii</i> Leaves.

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