High-Level Expression of Palmitoylated MPP1 Recombinant Protein in Mammalian Cells.

ABSTRACT: Our recent studies have pointed to an important role of the MAGUK family member, MPP1, as a crucial molecule interacting with flotillins and involved in the lateral organization of the erythroid plasma membrane. The palmitoylation of MPP1 seems to be an important element in this process; however, studies on the direct effect of palmitoylation on protein-protein or protein-membrane interactions in vitro are still challenging due to the difficulties in obtaining functional post-translationally modified recombinant proteins and the lack of comprehensive protocols for the purification of palmitoylated proteins. In this work, we present an optimized approach for the high-yield overexpression and purification of palmitoylated recombinant MPP1 protein in mammalian HEK-293F cells. The presented ap

SUBMITTER: Chytla A 

PROVIDER: S-EPMC8470630 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature