Optimizing Efficient RNAi-Mediated Control of Hemipteran Pests (Psyllids, Leafhoppers, Whitefly): Modified Pyrimidines in dsRNA Triggers.

ABSTRACT: The advantages from exogenously applied RNAi biopesticides have yet to be realized in through commercialization due to inconsistent activity of the dsRNA trigger, and the activity level of RNAi suppression. This has prompted research on improving delivery methods for applying exogenous dsRNA into plants and insects for the management of pests and pathogens. Another aspect to improve RNAi activity is the incorporation of modified 2'-F pyrimidine nucleotides into the dsRNA trigger. Modified dsRNA incorporating 32-55% of the 2'-F- nucleotides produced improved RNAi activity that increased insect mortality by 12-35% greater than non-modified dsRNA triggers of the same sequence. These results were repeatable across multiple Hemiptera: the Asian citrus psyllid (Diaphorina citri, Liviidae)

SUBMITTER: Hunter WB 

PROVIDER: S-EPMC8472347 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature