Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine.

ABSTRACT: Micro-blade design is an important factor in the cutting of single cells and other biological structures. This paper describes the fabrication process of three-dimensional (3D) micro-blades for the cutting of single cells in a microfluidic "guillotine" intended for fundamental wound repair and regeneration studies. Our microfluidic guillotine consists of a fixed 3D micro-blade centered in a microchannel to bisect cells flowing through. We show that the Nanoscribe two-photon polymerization direct laser writing system is capable of fabricating complex 3D micro-blade geometries. However, structures made of the Nanoscribe IP-S resin have low adhesion to silicon, and they tend to peel off from the substrate after at most two times of replica molding in poly(dimethylsiloxane) (PDMS). Our work de

SUBMITTER: Koppaka S 

PROVIDER: S-EPMC8472695 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature