Role of Gln79 in Feedback Inhibition of the Yeast γ-Glutamyl Kinase by Proline.

ABSTRACT: Awamori, the traditional distilled alcoholic beverage of Okinawa, Japan, is brewed with the yeast Saccharomyces cerevisiae. During the distillation process after the fermentation, enormous quantities of distillation residues containing yeast cells must be disposed of, and this has recently been recognized as a major problem both environmentally and economically. Proline, a multifunctional amino acid, has the highest water retention capacity among amino acids. Therefore, distillation residues with large amounts of proline could be useful in cosmetics. Here, we isolated a yeast mutant with high levels of intracellular proline and found a missense mutation (Gln79His) on the PRO1 gene encoding the γ-glutamyl kinase Pro1, a limiting enzyme in proline biosynthesis. The amino acid c

SUBMITTER: Nishimura A 

PROVIDER: S-EPMC8472793 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature