Prevalence and Molecular Characterization of Human Bocavirus Detected in Croatian Children with Respiratory Infection.

ABSTRACT: Human bocavirus (HBoV) 1 is considered an important respiratory pathogen, while the role of HBoV2-4 in clinical disease remains somewhat controversial. Since, they are characterized by a rapid evolution, worldwide surveillance of HBoVs' genetics is necessary. This study explored the prevalence of HBoV genotypes in pediatric patients with respiratory tract infection in Croatia and studied their phylogeny. Using multiplex PCR for 15 respiratory viruses, we investigated 957 respiratory samples of children up to 18 years of age with respiratory tract infection obtained from May 2017 to March 2021 at two different hospitals in Croatia. Amplification of HBoV near-complete genome or three overlapping fragments was performed, sequenced, and their phylogenetic inferences constructed. HBoV was detec

SUBMITTER: Ljubin-Sternak S 

PROVIDER: S-EPMC8473146 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature