Validation of a Genotype-Independent Hepatitis C Virus Near-Whole Genome Sequencing Assay.

ABSTRACT: Despite the effectiveness of direct-acting antiviral agents in treating hepatitis C virus (HCV), cases of treatment failure have been associated with the emergence of resistance-associated substitutions. To better guide clinical decision-making, we developed and validated a near-whole-genome HCV genotype-independent next-generation sequencing strategy. HCV genotype 1-6 samples from direct-acting antiviral agent treatment-naïve and -treated HCV-infected individuals were included. Viral RNA was extracted using a NucliSens easyMAG and amplified using nested reverse transcription-polymerase chain reaction. Libraries were prepared using Nextera XT and sequenced on the Illumina MiSeq sequencing platform. Data were processed by an in-house pipeline (MiCall). Nucleotide consensus sequences were al

SUBMITTER: Lapointe HR 

PROVIDER: S-EPMC8473162 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature