Finding Species-Specific Extracellular Surface-Facing Proteomes in Toxic Dinoflagellates.

ABSTRACT: As a sequel to our previous report of the existence of species-specific protein/peptide expression profiles (PEPs) acquired by mass spectrometry in some dinoflagellates, we established, with the help of a plasma-membrane-impermeable labeling agent, a surface amphiesmal protein extraction method (SAPE) to label and capture species-specific surface proteins (SSSPs) as well as saxitoxins-producing-species-specific surface proteins (Stx-SSPs) that face the extracellular space (i.e., SSSPs_Ef and Stx-SSPs_Ef). Five selected toxic dinoflagellates, Alexandrium minutum, A. lusitanicum, A. tamarense, Gymnodinium catenatum, and Karenia mikimotoi, were used in this study. Transcriptomic databases of these five species were also constructed. With the aid of liq

SUBMITTER: Chan KK 

PROVIDER: S-EPMC8473415 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature