Comparison of Different Labeling Techniques for the LC-MS Profiling of Human Milk Oligosaccharides.

ABSTRACT: Human milk oligosaccharides (HMOs) exhibit various biological activities for infants, such as serving as prebiotics, blocking pathogens, and aiding in brain development. HMOs are a complex mixture of hetero-oligosaccharides that are generally highly branched, containing multiple structural isomers and no intrinsic chromophores, presenting a challenge to both their resolution and quantitative detection. While liquid chromatography-mass spectrometry (LC-MS) has become the primary strategy for analysis of various compounds, the very polar and chromophore-free properties of native glycans hinder their separation in LC and ionization in MS. Various labeling approaches have been developed to achieve separation of glycans with higher resolution and greater sensitivity of detection. Here, we compa

SUBMITTER: Lang Y 

PROVIDER: S-EPMC8473617 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature