Genomic and Transcriptomic Characterization of Relapsed SCLC Through Rapid Research Autopsy.

ABSTRACT:

Introduction

Relapsed SCLC is characterized by therapeutic resistance and high mortality rate. Despite decades of research, mechanisms responsible for therapeutic resistance have remained elusive owing to limited tissues available for molecular studies. Thus, an unmet need remains for molecular characterization of relapsed SCLC to facilitate development of effective therapies.

Methods

We performed whole-exome and transcriptome sequencing of metastatic tumor samples procured from research autopsies of five patients with relapsed SCLC. We implemented bioinformatics tools to infer subclonal phylogeny and identify recurrent genomic alterations. We implemented immune cell signature and single-sample gene set enrichment analyses on tumor and normal transcriptome data from autopsy

SUBMITTER: Chen HZ 

PROVIDER: S-EPMC8474405 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature