Spatially Resolved Proteomic Analysis of the Lens Extracellular Diffusion Barrier.

ABSTRACT:

Purpose

The presence of a physical barrier to molecular diffusion through lenticular extracellular space has been repeatedly detected. This extracellular diffusion barrier has been proposed to restrict the movement of solutes into the lens and to direct nutrients into the lens core via the sutures at both poles. The purpose of this study is to characterize the molecular components that could contribute to the formation of this barrier.

Methods

Three distinct regions in the bovine lens cortex were captured by laser capture microdissection guided by dye penetration. Proteins were digested by Lys C and trypsin. Mass spectrometry-based proteomic analysis followed by gene ontology and protein interaction network analysis was performed.

Results

Dye penetration showed that f

SUBMITTER: Wang Z 

PROVIDER: S-EPMC8475287 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature