Uncovering the Association Between m⁵C Regulator-Mediated Methylation Modification Patterns and Tumour Microenvironment Infiltration Characteristics in Hepatocellular Carcinoma.

ABSTRACT: Background: 5-Methylcytosine (m⁵C) plays essential roles in hepatocellular carcinoma (HCC), but the association between m⁵C regulation and immune cell infiltration in HCC has not yet been clarified. Methods: In this study, we analysed 371 patients with HCC from The Cancer Genome Atlas (TCGA) database, and the expression of 13 m⁵C regulators was investigated. Additionally, gene set variation analysis (GSVA), unsupervised clustering analysis, single-sample gene set enrichment analysis (ssGSEA), correlation analysis, and immunohistochemical (IHC) staining were performed. Results: Among the 371 patients, 41 had mutations in m⁵C regulators, the frequency of which was 11.26%. Compared with normal hepatic tissues, the expression of

SUBMITTER: Gu X 

PROVIDER: S-EPMC8475949 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature