Identification and validation of circulating miRNAs as potential new biomarkers for severe liver disease in patients with leptospirosis.

ABSTRACT:

Background

Leptospirosis, a global zoonotic infectious disease, has various clinical manifestations ranging from mild self-limiting illness to life-threatening with multi-organ damage, including liver involvement. This study was aimed at identifying circulating microRNAs (miRNAs) as novel biomarkers for predicting severe liver involvement in patients with leptospirosis.

Methods

In a discovery set, 12 serum samples of patients with anicteric and icteric leptospirosis at initial clinical presentation were used for miRNA profiling by a NanoString nCounter miRNA assay. In a validated cohort, top candidate miRNAs were selected and further tested by qRT-PCR in serum samples of 81 and 16 individuals with anicteric and icteric leptospirosis, respectively.

Results

The discover

SUBMITTER: Chuaypen N 

PROVIDER: S-EPMC8476044 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature