Zinc accelerates respiratory burst termination in human PMN.

ABSTRACT: The respiratory burst of phagocytes is essential for human survival. Innate immune defence against pathogens relies strongly on reactive oxygen species (ROS) production by the NADPH oxidase (NOX2). ROS kill pathogens while the translocation of electrons across the plasma membrane via NOX2 depolarizes the cell. Simultaneously, protons are released into the cytosol. Here, we compare freshly isolated human polymorphonuclear leukocytes (PMN) to the granulocytes-like cell line PLB 985. We are recording ROS production while inhibiting the charge compensating and pH regulating voltage-gated proton channel (H_V1). The data suggests that human PMN and the PLB 985 generate ROS via a general mechanism, consistent of NOX2 and H_V1. Additionally, we advanced a mathematical model based on the biophysical properties of NOX2 and H_V1. Our results strongly suggest the essential interconnection of H_V1 and NOX2 during the respiratory burst of phagocytes. Zinc chelation during the time course of the experiments postulates that zinc leads to an irreversible termination of the respiratory burst over time. Flow cytometry shows cell death triggered by high zinc concentrations and PMA. Our data might help to elucidate the complex interaction of proteins during the respiratory burst and contribute to decipher its termination.

SUBMITTER: Droste A 

PROVIDER: S-EPMC8476447 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature